nstitut Neuromyogene (INMG), Physiopathology and Genetics of Neuron and Muscle (PGNM), CNRS UMR5261, INSERM U1315
Université Claude Bernard Lyon 1 - Campus Rockefeller
Team
Muscle nuclear and cytoskeleton architecture
Members
Gaelle Boncompain - gaelle.boncompain@univ-lyon1.fr
Keywords
Secretory pathway, intracellular compartments, Golgi apparatus, hiPSC
Description
Secretory protein transport is necessary to fulfil essential cellular functions, enabling communication between cells, tissue maintenance and specialized functions.
Differentiated tissues display distinct secretory needs, requiring adaptations of the transport routes and of the Golgi apparatus, the cellular central hub for secretion.
Using human induced pluripotent stem cells (hiPSC)-derived models, we investigate the mechanisms of intracellular protein transport in differentiated cells (cardiomyocytes, chondrocytes and skeletal muscle cells) using real-time quantitative fluorescence microscopy, genome-editing and organelle- specific proteomics
Biological models
Human induced pluripotent stem cells (hiPSC), hiPSC-cardiomyocytes, hiPSC-chondrocytes, myotubes